ABSTRACT
Objective: The intension of the present study includes fabrication and optimization of mouth dissolving film loaded with Chlorothalidone by solvent evaporation techniques using two components and their three levels as multilevel Categoric design. Methods: Major problem associated with the development of film loaded with BCS class II drug is to increase its solubility. Here the Chlorothalidone solubility achieved by co-solvents, such as methanol. After dissolving the drug in co-solvent, this drug solution is poured into an aqueous dispersion of Hydroxypropyl Methylcellulose E5 (HPMC E5) and Polyethylene glycol 400 (PEG 400). The two independent variables selected are factor A (concentration of HPMC E5) and factor B (concentration of PEG 400) was selected on the basis of preliminary trials. The percentage drug release (R1), Disintegration time in sec (R2) and folding endurance (R3) were selected as dependent variables. Here HPMC E5 used as a film former, PEG 400 as plasticizer, mannitol as bulking agent, Sodium starch glycolate as a disintegrating agent, tween 80 as the surfactant, tartaric acid as saliva stimulating agent, sodium saccharin as a sweetener and orange flavour etc. These fabricated films were evaluated for physicochemical properties, disintegration time and In vitro drug release study. Results: The formulation F6 has more favorable responses as per multilevel categoric design is % drug release about 98.95 %, average disintegration time about 24.33 second and folding endurance is 117. Thus formulation F6 was preferred as an optimized formulation. Conclusion: The present formulation delivers medicament accurately with good therapeutic efficiency by oral administration, this mouth dissolving films having a rapid onset of action than conventional tablet formulations.
ABSTRACT
Introduction: Measurement of prolactin and thyroid hormones, especially thyroid stimulating hormone (TSH), has been considered an important component of infertility work up in women. Thyroid dysfunctions interfere with numerous aspects of reproduction and pregnancy. Aim: To correlate thyroid hormones level with FSH, LH and prolactin in infertility in the reproductive age group women. Materials and methods: This study includes 100 infertile women who attended infertility clinic of our institute along with 50 fertile women as a control group between age group of 20 to 40 years. Out of 100 infertile women, 70 were of primary infertility and 30 of secondary infertility. Thyroid hormones and infertility hormones level is measured from all participants by chemiluminance immunoassay. Results: Prolactin and TSH were positively correlated with each other. They were also negatively correlated with LH, FSH and T3 in infertile groups. Therefore we can say that hyperprolactinemia and hypothyroidism plays key role in etiopathogenesis of infertility. Conclusion: There was a higher crude prevalence of hypothyroidism and hyperprolactinemia in the infertile women as compared to the fertile ones in the control group.